Light and Electron Microscopical Localization of Lysosomal Acid Hydrolases in Bivalve Haemocytes by Enzyme Cytochemistry.

Lysosomal enzyme cytochemistry in acute leukaemia.

A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classification of cell type and is superior in this respect to the other lysosomal enzymes ...

Lysosomal enzyme cytochemistry of blood neutrophils.

Patients with bacterial infection may show altered membrane permeability of the primary azurophilic lysosomes of blood neutrophils. A new enzyme cytochemical technique, sensitive to increased membrane permeability caused by contact of neutrophils with acetone, saponin, low pH, Streptolysin O, bacteria, and nylon wool, has been developed. The method is of potential value as a screening test for ...

Lysosomal Acid Hydrolases in Mice Infected with Bcg* by Kazuhisa

Infection of laboratory animMs with BCG, an attenuated strain of tubercle bacilli, results in functional changes of the RES x, especially enhanced activity. The heightened phagocytic capacity may well contribute toward resistance of such animals to homologous and heterologous virulent infection (2). One of the consequences of altered activity of the RES induced by infection with BCG is the well...

Localization of Acid hydrolases in protoplasts: examination of the proposed lysosomal function of the mature vacuole.

The development of techniques to isolate and purify relatively large quantities of intact vacuoles from mature tissues permits direct biochemical analysis of this ubiquitous mature plant cell organelle. Vacuoles and a fraction enriched in soluble cytoplasmic constituents were quantitatively prepared from Hippeastrum flower petal protoplasts. Vacuolar lysate and soluble cytoplasmic fractions wer...

Light microscopical diagnosis of rabies